Charge microheterogeneity of myelin basic protein in murine brain by Anthony Edward Palma

Cover of: Charge microheterogeneity of myelin basic protein in murine brain | Anthony Edward Palma

Published by National Library of Canada = Bibliothèque nationale du Canada in Ottawa .

Written in English

Read online

Edition Notes

Book details

SeriesCanadian theses = Thèses canadiennes
The Physical Object
FormatMicroform
Pagination2 microfiches.
ID Numbers
Open LibraryOL18567388M
ISBN 100612287629
OCLC/WorldCa46574525

Download Charge microheterogeneity of myelin basic protein in murine brain

In murine brain. myelin basic protein represents a highly heterogeneous family of proteins consisting of several isoforms and charge isomers (or components).

On alkaline- urea slab gels. murine MBP resolved into two major groups of isomers: the (slow migrating group) and 14 kDa (fast migrating group) isoforms. Following isolation byAuthor: Anthony Edward Palma. Charge Charge microheterogeneity of myelin basic protein in murine brain book of Myelin Basic Protein: Structure and Interactions with Membranes, Nucleotide Analogues, and Calmodulin.

Conceived and designed the experiments: CW UN JB JP PK. Performed the experiments: CW UN JB MM PB PK. Analyzed the data: CW UN JB MM PB JP by: Myelin basic protein (MBP), a major protein of the myelin sheath, is encoded by the Golli (genes of oligodendrocyte lineage)-MBP gene in myelinating glia and immune cells.1 Several size isoforms of classic MBP are formed by differential splicing of a single mRNA transcript.

The major MBP isoform in the adult human and bovine CNS is kDa protein that plays a structural role in maintaining myelin by: 2. The basic protein of bovine central nervous system myelin contains a single polypeptide chain of amino acids. Multiple components of basic protein have been observed on disc gel electrophoresis and ion exchange chromatography at alkaline pH, but the basis of the microheterogeneity has not been established.

Moscarello MA, Brady GW, Fein DB, Wood DD, Cruz TF. The role of charge microheterogeneity of basic protein in the formation and maintenance of the multilayered structure of myelin: a possible role in multiple sclerosis. J Neurosci Res. ; 15 (1)– Sorg BA, Smith MM, Campagnoni by: The role of charge microheterogeneity of basic protein in the formation and maintenance of the multilayered structure of myelin: A possible role in multiple sclerosis.

Journal of Neuroscience Research15 (1), DOI: /jnr Literature by:   Charge microheterogeneity of myelin basic protein is known to affect its conformation and function. Here, the citrullinated myelin basic protein charge iso Since levels of component-8 are increased in multiple sclerosis brain, the increased susceptibility of component-8 to proteolytic digestion may play a role in the pathogenesis of multiple by: Abstract.

Myelin basic protein (MBP) has been known since the mid ’s. Its primary structure was reported by two groups independently, Eylar et al. reported on the bovine MBP while Carnegie reported on the human MBP (Eylar et al., ; Carnegie, ).Cited by:   Basis of microheterogeneity of myelin basic protein.

Chou FC, Chou CH, Shapira R, Kibler RF. The basic protein of bovine central nervous system myelin contains a single polypeptide chain of amino by: The recombinant kDa charge isoform Charge microheterogeneity of myelin basic protein in murine brain book murine myelin basic protein (rmMBP) is unmodified posttranslationally and was used to study the effects of deimination, i.e., the conversion of arginyl to citrullinyl residues, on the protein's interactions with itself and with by:   In contrast, MBPs from day-old and adult normal mouse brain displayed extensive charge microheterogeneity, having at least eight components.

A 32 kDa MBP was the major isoform observed on immunoblots of acid-soluble protein from 6-day-old and day-old normal and day-old jimpy mouse by: A recombinant hexahistidine-tagged kDa isoform of murine myelin basic protein has been characterized biochemically and immunogenically, by mass spectrometry, by circular dichroism under various conditions (in aqueous solution, with monosialoganglioside G M1, and in 89% 2-propanol), and by transmission electron microscopy.

The preparations of this protein indicated Cited by: Components of basic protein were separated on the basis of net charge. Differences in ionic interactions of components of basic protein in a two-phase partition system with beef brain phosphatidyl serine were small, but there was a tendency for less charged components to require less lipid to achieve the same degree of solubilization into the chloroform by: Myelin basic protein: a multifunctional protein.

Cell Mol Life Sci Article Literature Review in Cellular and Molecular Life Sciences CMLS 63(17) Author: Joan Boggs. The classic myelin basic protein (MBP) splice isoforms range in nominal molecular mass from 14 to kDa, and arise from the gene in the oligodendrocyte lineage (Golli) in maturing oligodendrocytes.

Phosphorylation is one of a number of post-translational modifications resulting in charge microheterogeneity of myelin basic protein (MBP). This phosphorylation is claimed to destabilise the compact myelin sheath by decreasing the interaction of membrane bilayers, thereby creating or maintaining pockets of by: 7.

Myelin basic protein is a multifunctional protein whose primary role is to adhere membranes of the myelin sheath. There are various isoforms that Author: Joan Boggs.

The pathogenesis of MS is unknown. In our studies, we have demonstrated an important role for citrullinated myelin basic protein (MBP).

The accompanying loss of positive charge compromises the ability of MBP to interact with the lipid bilayer. The conversion of arginine to citrulline in brain is carried out by an enzyme peptidyl arginine deiminase (PAD) by: The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS.

They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli.

Of these candidate autoantigens, myelin basic protein (MBP), the second most abundant protein in myelin, has been widely studied (BoggsCampagnoniCampagnoniHarauz ) and its importance to the immunopathogenesis of MS is extensively reviewed elsewhere (LuttonSospedraSteinman ). MBP and its peptides are Cited by: Post-translational Modifications of Chicken Myelin Basic Protein Charge Components Article in Neurochemical Research 34(2) August with 5 Reads How we measure 'reads'.

Charge microheterogeneity of myelin basic protein is known to affect its conformation and function. Here, the citrullinated myelin basic protein charge isomer, component-8. Myelin basic protein (MBP) plays an important structural and functional role in the neuronal myelin sheath.

Translated MBP exhibits extreme microheterogeneity with numerous alternative splice variants (ASVs) and post-translational modifications (PTMs) reportedly tied to central nervous system maturation, myelin stability, and the pathobiology of various de- and dys Cited by: 4.

Myelin basic protein (MBP) plays an important structural and functional role in the neuronal myelin sheath. Translated MBP exhibits extreme microheterogeneity with numerous alternative splice variants (ASVs) and post-translational modifications (PTMs) reportedly tied to central nervous system maturation, myelin stability, and the pathobiology of various de- and Cited by: 4.

Myelin basic protein (MBP) is a protein believed to be important in the process of myelination of nerves in the nervous system. The myelin sheath is a multi-layered membrane, unique to the nervous system, that functions as an insulator to greatly increase the Aliases: MBP, entrez, myelin basic protein.

Abstract. In the second edition of the book Neurochemistry, Rossiter () stated, “Since pure ‘myelin’ is not available for direct chemical analysis, the neurochemist has been compelled to deduce the constituents for myelin from such analyses as are practicable.”The available analyses were extensive.

Qualitative information had been accumulating from histological staining Cited by:   Myelin localization of peptidylarginine deiminases 2 and 4: comparison of PAD2 and PAD4 activities The number of gold particles in myelin from MS brain was Effect of bovine basic protein Cited by:   Myelin basic protein (MBP) is the second most abundant protein of central nervous system (CNS) myelin (after the proteolipid protein), representing about 30 % of the total myelin protein and about 10 % of myelin by weight.

It is also present in peripheral nervous system (PNS) myelin but as a lower percentage of the total : Hardcover. Alternative Name Myelin basic protein (MBP Antibody Abstract) Background Myelin basic protein(MBP) is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the central nervous system and the peripheral nervous system, respectively.

Myelin basic protein, MBP, Myelin A1 protein, Myelin membrane encephalitogenic protein. Introduction MBP is a major component of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system, although, MBP-related transcripts are also present in the bone marrow and the immune system.

Abstract: Myelin basic protein (MBP) phosphorylation is a complex regulatory process that modulates the contribution of MBP to the stability of the myelin research has demonstrated the modulation of MBP phosphorylation by mitogen‐activated protein kinase (MAPK) during myelinogenesis and in the demyelinating disease multiple Cited by: Myelin basic protein (MBP) is an kDa protein encoded by the gene mapped to human chromosome 18q22qter.

MBP is a major component of myelin sheath and is highly expressed in central nervous system (CNS). Application Myelin basic protein antigen from human brain has been used in enzyme-linked immunosorbent spot assays. MBP Antibody Staining Protocol for Immunohistochemistry.

Description: Myelin basic protein (MBP) is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the central nervous system and the peripheral nervous system, respectively.

It is most abundant in hemopoietic system and contains seven exons distributed over kb. Myelin basic protein (MBP) charge variants show different sphingomyelin-mediated interactions with myelin-like lipid monolayers.

Biochimica et Biophysica Acta (BBA) - Biomembranes(2), DOI: / Arne Raasakka, Petri by: : Myelin basic Protein antibody - myelin basic protein, 25 μl supplied: Industrial & Scientific.

Myelin protein zero (P0, MPZ) is a single membrane glycoprotein which in humans is encoded by the MPZ gene. P0 is a major structural component of the myelin sheath in the peripheral nervous system (PNS). Myelin protein zero is expressed by Schwann cells and accounts for over 50% of all proteins in the peripheral nervous system, making it the most common protein Aliases: MPZ, CHM, CMT1, CMT1B.

Myelin is a lipid-rich (fatty) substance that surrounds nerve cell axons (the nervous system’s wires) to insulate them and increase the rate at which information (encoded as electrical impulses) is passed along the myelinated axon can be likened to an electrical wire (the axon) with insulating material (myelin) around it.

However, unlike the plastic covering on an electrical wire FMA: Experimental autoimmune encephalomyelitis, sometimes experimental allergic encephalomyelitis (EAE) is an animal model of brain is an inflammatory demyelinating disease of the central nervous system (CNS).

It is mostly used with rodents and is widely studied as an animal model of the human CNS demyelinating diseases, including multiple sclerosis and acute.

CSF myelin basic protein is a test to measure the level of Myelin. Myelin is an insulating layer, or sheath that forms around nerves, including those in the brain and spinal cord. It is made up of protein and fatty Read Article Now Book Mark Article.

The CSF is the clear liquid that surrounds the brain and spinal cord. Western blot analysis of extracts from mouse brain and C2C12 cells using Myelin Basic Protein (D8X4Q) XP ® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb # (lower). Immunohistochemical analysis of a paraffin-embedded human Alzheimer's brain using Myelin Basic Protein (D8X4Q) XP ® Rabbit ry: Primary Antibodies.

@article{osti_, title = {Structure and expression of a novel compact myelin protein – Small VCP-interacting protein (SVIP)}, author = {Wu, Jiawen and Peng, Dungeng and Voehler, Markus and Sanders, Charles R. and Center for Structural Biology, Vanderbilt University and Li, Jun and Tennessee Valley Healthcare System}, abstractNote = {Highlights: •SVIP (small .Susceptibility to multiple sclerosis is associated with the human histocompatibility leukocyte antigen (HLA)-DR2 (DRB1*) haplotype.

The structure of HLA-DR2 was determined with a bound peptide from human myelin basic protein (MBP) that is immunodominant for human MBP-specific T by: Another myelin-related homologous protein present in many vertebrates is M6B.

Although it is a general a proteolipid protein used in various processes, its use in oligodendrocytes and in part of myelin-enriched membranes (Schweitzer et al., ) qualifies it for discussion as a myelin protein.

61446 views Wednesday, December 2, 2020